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. 2013 Dec 19;9(12):e1003792. doi: 10.1371/journal.ppat.1003792

Figure 1. BgLBP/BPI1 is expressed in the albumen gland and is the major protein of B. glabrata egg masses.

Figure 1

(A) Relative expression levels of BgLBP/BPI1 transcripts in the albumen gland (AG), hepatopancreas (Hp), Headfoot (HF), gonads (G), digestive tract (DT) and circulating hemocytes (HC) determined using real-time quantitative PCR (data from 3 independent experiments). Expression has been normalized to expression of S19 and EEF1-α reference genes. (B) Representative western blot showing the content in BgLBP/BPI1 protein in 15 µg total protein from the corresponding snail tissues (legend as in (A). (C) Typical live 2-days old B. glabrata egg mass with eggs (e) containing embryos (em) observed under a phase-contrast reversed microscope. (D) Total egg mass protein content as revealed by silver staining (ss) and egg mass BgLBP/BPI1 revealed by western blot (wb) using a custom-made antibody raised against two BgLBP/BPI1 peptides.