Figure 6. Injection of BgLBP/BPI1 dsRNA results in a substantial decrease in BgLBP/BPI1 protein in the albumen gland and in egg masses.

(A) Western blots of albumen glands from snails at 9 days and 12 days post injection (Days PI) of dsRNA of luciferase (non-relevant dsRNA) (dsLuc) or BgLBP/BPI1 (dsBgLBP/BPI1). Actin was used as an endogenous control for protein loads. (B) Western blots of egg masses laid by snails at 18 and 21 days post injection of dsRNAs. Note that control for actin or total protein contents were not appropriate for egg masses and that protein loads have been standardized towards egg mass dry weights. Western Blots were performed using a custom-made antibody raised against two BgLBP/BPI1 peptides.