Figure 9. Experimental testing of computational predictions.

Qualitative analysis of networks from in silico simulations nominates PLK1 and Cyclin B1 as potential targets to kill RAF inhibitor resistant melanoma cells. A validation experiment with the PLK1 inhibitor BI2536 shows extensive growth inhibition in SKMEL133 cells (Cell viability IC50 = 5.8 nm). PLK1 inhibitor is a pure prediction of the approach and was not included in the experimental drug set.