Table 1. Strains and plasmids.

Strain	Genotype/relevant properties	Source/reference
DH10B	F−, mcrA, Δ(mrr-hsdRMS-mcrBC), Φ80dlacZΔM15, ΔlacX74, endA1, recA1, deoR, Δ(ara,leu)7697, araD139, galU, galK, nupG, rpsL	[55]
MC1061	araD139, Δ(ara-leu)7679, ΔlacX74, galU, galK, rpsL, thi, sdR2, mcrB	[56]
MG1655	F−, λ−	[57]
DLT1900	MC1061 ΔaraFGH, Ωpcp18::araE533	[9]
DLT2202	MG1655 ΔaraFGH, Ωpcp18::araE533	This work
DLT2583	DLT2202 Δ(araBAD')-Ω(para-tetR::gfp)	This work
DLT2687	DLT1900 ΔlacZ Ω(plac-sopA::xfp)	This work
DLT2740	DLT2583 ΔlacZ Ω(plac-sopA::xfp)	This work
DLT2721	DLT2687 pAM238	This work
DLT2722-6	DLT2687 pDAG209, pYAS47, -64, -65, -66, -67	This work
DLT2891	DLT2583 pDAG848, pAM238	This work
DLT2734-8	DLT2583 pDAG848, pYAS47, -64, -65, -66, -67	This work
DLT2867	DLT2740 pDAG848, pAM238	This work
DLT2741-2	DLT2740 pDAG848, pYAS47, -64	This work
Plasmid	Relevant characteristics
pAM238	lacOPZ'Ωmcs in EcoRI-HindIII interval of pGB2, aadA +	[58]
pDAG114	repFIA, ccdB −, resD +, rsfF +, cat	[59]
pDAG173	pDAG114 with sopAB control replaced by pLtetO	[49]
pDAG209	pDAG114 ΔsopAB	[59]
pDAG218	pDAG114 with lacZ inserted between sopB and sopC	This work
pDAG848	pDAG209, tetO array	This work
pDAG781	pDAG218 sopB-R36A	This work
pDAG782	pDAG218 sopB-R36K	This work
pDAG783	pDAG218 sopB-R42A	This work
pDAG784	pDAG218 sopB-R42K	This work
pYAS29	pDAG173 sopB-R42A	This work
pYAS37	pDAG173 sopB-R42K	This work
pYAS43	pDAG173 sopB-R36A	This work
pYAS45	pDAG173 sopB-R36K	This work
pYAS47	pAM238 plac-sopAB	This work
pYAS64	pYAS47 sopB-R42A	This work
pYAS65	pYAS47 sopB-R42K	This work
pYAS66	pYAS47 sopB-R36A	This work
pYAS67	pYAS47 sopB-R36K	This work
pYAS70	pYAS47 Δ(sopA 115–358)	This work
pYAS71	pYAS64 Δ(sopA 115–358)	This work
pYAS81	pYAS47 sopB-R36A, R42A	This work
pJYB259	pYAS47 sopB::mVenus	This work
pJYB260	pYAS64 sopB::mVenus	This work