Table 1. Differences in differentiation potential between wild OBNS/PC, OBNS/PC-GFP, and –OBNS/PC-GFP-hNGF using GFAP, MAP2, β-TubulinIII, NG2, and O4 immunocytochemistry markers.
Cell Type | Control OBNS/PC | GFP- OBNS/PC | GFP-NGF-OBNS/PC |
---|---|---|---|
GFAP +ve (Astrocyte marker) | 65-75% | 60-70% | 45-55% |
MAP2 +ve (Mature neurons) | 8% | 15% | 25-30% |
β-TubulinIII +ve (Immmature neurons) | 3% | 8% | 6% |
NG2+ve (Immature oligodendrocytes) | 8% | 7-8% | 14% |
NG2 +ve +PDGFAA+T3 | 15% | 16% | 25% |
O4+ve (Immature oligodendrocytes) | Negative | negative | negative |