Figure 1.

Metformin alleviates hepatic steatosis in ob/ob mice. (A) Hematoxylin and eosin (H&E) staining of liver sections from wild-type (WT; C57BL/6, control) or ob/ob mice treated without or with metformin for 4 weeks (scale bar, 50 μm). (B) Representative frozen tissue sections of livers stained with Oil Red O to demonstrate the reduction in lipid droplets (scale bar, 100 μm). (C) Body weight of the experimental mice is shown. (D) Hepatic triglyceride (TG) concentration was quantified using thin layer chromatography. All data are expressed as the means ± SEM (n=8) for each group. ^**P<0.05. (E) Hepatic adipose differentiation-related protein (ADRP) protein level was detected by western blot analysis, α-tubulin was used as an internal control for equal loading of the samples. (F) Hepatic ADRP mRNA expression was assessed by real-time PCR. Data shown are the means ± SEM of representative experiments performed in triplicate. ^*P<0.05.