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. 2013 Nov 19;33(1):51–58. doi: 10.3892/ijmm.2013.1560

Figure 5.

Figure 5

Effect of treatment with siRNA targeting adipose differentiation-related protein (ADRP) lipid accumulation in primary hepatocytes. Primary hepatocytes were infected with an adenovirus carrying ADRP siRNA for 6 h and then incubated with 200 μM oleate and 500 μM metformin for 24 h. (A) BODIPY staining of cells infected with ADRP siRNA treated with metformin (green staining). Nuclei were stained with Hoechst 33258 (blue staining) (scale bar, 15 μm). (B) Concentration of triglycerides (TG) was determined in si-ADRP-infected cells treated with metformin, compared with the si-scramble (control)-infected cells. Data are presented as the means ± SEM (n=4), *P<0.05. (C) Western blot analysis revealed that the expression level ADRP in the cells infected with si-ADRP or si-scramble and treated with metformin; α-tubulin was used as the control. (D) The relative mRNA level of ADRP is also shown. The relative mRNA level in the control group was designated as 1.0. n=4.