Figure 3. Fluorescence co-labeling of marker combinations.

. The markers are labeled with Alexa568 (red), Alexa488 (green) and DAPI (blue). Marker combinations are indicated above each pictogram. Dotted-line circles indicate examples of single labeled neurons, solid-line circles indicate examples of double labeled neurons. The letter “U” indicates examples of unlabeled neurons, some with lipofuscin. Scale bars represent 50 µm.