Table 1. Characteristics and frequency of neuronal markers analyzed.
Antigen recognized by antibody | Expected cellular localizationa | Proposed characteristics of labeled neurons | Data from animal models | % labeled neurons (number of counted neurons (present study)) | |
Ret | GDNF-family ligand receptor tyrosine kinase | Membrane protein, also seen as cytoplasmic and Golgi hn [34] | Small diameter neurons, especially nociceptors [51] | Labels neurons equivalent to KH10 antibody in mouse [23] | 26.93 (2306) |
TrkA | High-affinity nerve growth factor receptor tyrosine kinase | Cytoplasmic and Golgi hn [52] Plasma membrane ho [53] | Small diameter neurons, especially nociceptors [51] | Labels neurons equivalent to A5 antibody in mouse [23] | 61.82 (2499) |
nNOS | Neuronal nitric oxide synthase | Cytoplasmic hn [54] | Prevalence in ophthalmic branch of TG [20] | NO can inhibit HSV-1 replication [21] | 33.13 (2792) |
RT97 | Phosphorylated 200kDa neurofilament | Cytoplasmic an [19], [55] | Larger A-type neurons [45] | 39.26 (2127) | |
CGRP | Calcitonin gene-related peptide, a vasodilator and pain mediator | Cytoplasmic hn [56] | Smaller sized neurons [20] | Found throughout the TG of mice [20], and innervate the gingiva in rats [33] | 27.16 (2003) |
Peripherin | Type III intermediate filament | Cytoplasmic hn [57] | Neurons of the peripheral nervous system and small nerve fibers of the sensory ganglia [58] | Upregulated in response to nerve injury and proinflammatory cytokines [59] | 40.87 (2109) |
a: References are marked according to whether the experiments were performed in humans (h) or animals (a), and neurons (n) or other cell types (o).