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. 2013 Dec 19;8(12):e84073. doi: 10.1371/journal.pone.0084073

Figure 2. Obatoclax and ABT-737 induce regulation of Bcl-2 family proteins.

Figure 2

A. Upregulation of BH3-only proteins and anti-apoptotic Bcl-2 family proteins occurs in response to Obatoclax. Whole cell lysates of Mel-RM and MM200 cells were subjected to Western blot analysis following treatment with Obatoclax (1µM) or ABT-737 (10µM) for indicated periods. Data are representative of three individual experiments.

B. Knockdown of Mcl-1 increases cell sensitivity to ABT-737. Mel-RM and MM200 cells were transfected with either control or Mcl-1 siRNA before treatment with ABT-737 (10μM) for 24 hours. Cells were then subjected to measurement of apoptosis by the propidium iodide method. Columns, mean of three individual experiments; bars, SE. Knockdown of Mcl-1 was confirmed by Western blotting. Photographs representative of cell population at 24 hours are displayed and are representative of three individual experiments.