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. 2013 Dec 19;8(12):e84073. doi: 10.1371/journal.pone.0084073

Figure 6. Induction of autophagy by BH3 mimetics.

Figure 6

A. Mel-RM and MM200 cells were treated with Obatoclax (1µM) or ABT-737 (10µM) for indicated times. Whole cell lysates were collected and subjected to Western blot analysis for LC3 protein levels. The cytosolic form, LC3-I, is expressed as a 16kDa protein, which is lipidated and bound to autophagosome membranes, resolving as a 14kDa form, LC3-II. Data are representative of three individual experiments.

B. Mel-RM and MM200 cells were transfected with either control, Bcl-2 or Mcl-1 siRNA before treatment with either Obatoclax (1µM) or ABT-737 (10µM) as indicated for 16 hours. Whole cell lysates were collected and subjected to Western blot analysis for Mcl-1 and Bcl-2, to confirm knockdown efficiency, and LC3 and p62 to investigate induction of autophagy. Data are representative of two individual experiments.