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. 2013 Dec 19;8(12):e84436. doi: 10.1371/journal.pone.0084436

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© 2013 Lam et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) RT-PCR analysis of hsp70, hsp27 and splicing of xbp1 (p, pre-sliced; s, spliced) in control (cntl) or heat shock (HS) larvae. (B-C) RT-PCR analysis of the enrichment of RNA from neutrophils (lyz:TRAP), macrophages (mpeg1:TRAP) or epithelial cells (krt4:TRAP) from Tg(lyz:EGFP-L10a), Tg(mpeg1:EGFP-L10a) or Tg(krt4:EGFP-L10a) larvae, respectively. Whole larvae RNA (WT) or translating ribosome affinity purification (TRAP) RNA was used and specific markers for neutrophils (mpx), macrophages (mpeg1), epithelial cells (krt4) and muscle cells (myoD) were tested. (D) Heat shock (HS) induced expression of hsp70, hsp27 and splicing of xbp1 (p, pre-sliced; s, spliced) in neutrophils (lyz:TRAP), macrophages (mpeg1:TRAP) and epithelial cells (krt4:TRAP). Expression of hsp27 persisted at 3 hours post heat shock (3 hpHS) while expression of hsp70 and splicing of xbp1 returned to control levels at 3 hpHS. Data are representative of at least two experiments.