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. 2013 Dec 19;8(12):e84436. doi: 10.1371/journal.pone.0084436

Figure 5. Expression of Hsp27, Hsp70 or spliced Xbp1 in neutrophils or epithelial cells does not alter neutrophil recruitment.

Figure 5

(A-C) Quantification of neutrophils at the otic vesicle (ear) of larvae at 2 hours post infection (hpi) with 100 CFU S.i. ear infection. Experiments were performed on the larvae of transgenic lines Tg(lyz:hsp70-2A-EGFP) x Tg(krt4:hsp70-2A-mCherry) (A), Tg(lyz:hsp27-2A-mCherry) (B) or Tg(lyz:xbp1s-2A-EGFP) x Tg(krt4:xbp1s-2A-mCherry) (C) at 3 dpf. Controls (cntl) were siblings with no transgene expression. Expression of Hsp27, Hsp70 and spliced Xbp1 (Xbp1s) did not have a significant effect on neutrophil recruitment to ear infection. (D-H) Quantification of neutrophils at tail fin wounds at 4 hpw. Experiments were performed on Tg(lyz:hsp70-2A-EGFP) (D), Tg(krt4:hsp70-2A-mCherry) (E), Tg(lyz:hsp27-2A-mCherry) (F), Tg(lyz:xbp1s-2A-EGFP) (G) or Tg(krt4:xbp1s-2A-mCherry) (H) larvae at 3 dpf. Controls (cntl) were siblings with no transgene expression. Expression of Hsp27, Hsp70 and spliced Xbp1 (Xbp1s) did not have a significant effect on neutrophil numbers at the wound at 4 hpw. Data are representative of at least two experiments.