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. Author manuscript; available in PMC: 2014 Nov 6.
Published in final edited form as: J Am Chem Soc. 2013 Nov 6;135(44):10.1021/ja408909h. doi: 10.1021/ja408909h

Figure 8.

Figure 8

Detection of CSE-produced H2S using CLSS-2. Conditions: Absence of enzyme (white), 10 μg CSE (light grey), 20 mM Hcy, 20 mM BCA, 25 μM pyruvate, 25 μM NH3; incubated at in 3.0 mL buffer at 37 °C for 48 h prior to detection with 50 μM CLSS-2. Comparison with C6 cell lysates containing 2 × 106 cells (dark grey). Each data point represents the mean ± SE derived from at least 3 independent experiments; * indicates p < 0.005 and ** indicates p < 0.001.