Figure 5. Analysis of peritoneal lymphocyte subsets from mice injected with the anti-PD-1/CD137 combination.

Mice (3/group) transplanted i.p. with 3 × 10⁶ ID8 cells 10 day earlier were injected i.p. twice at 4 days interval with 0.5 mg of control, anti-CD137, anti-PD-1 or anti-PD-1/CD137 mAb. Two weeks later, peritoneal lavage from treated mice was analyzed by flow cytometry for the percentage and phenotype of peritoneal lymphocytes. The percentages of CD3⁺, CD4⁺, CD8⁺ and CD19⁺ lymphocytes in peritoneal lavage and CD44⁺CD62L^- effector/memory cells in CD4⁺ and CD8⁺ T cells are shown in (A) and (B) respectively. The percentage of PD-1⁺TIM-3⁺ and PD-1^-TIM-3⁺ cells in peritoneal CD4⁺ and CD8⁺ T cells are shown in (C) with representative dotplots in Figure S2. Data are presented as M±SEM from 3 mice of each group and are representative of 2 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, PD-1 or CD137 mAb compared with control mAb, PD-1/CD137 mAb compared with control and single mAb.