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. Author manuscript; available in PMC: 2014 May 18.
Published in final edited form as: Nat Commun. 2013;4:2805. doi: 10.1038/ncomms3805

Figure 5. Reduced weight and fibre cross-sectional area in dnOrai1 transgenic mice.

Figure 5

(a) Postnatal growth curves (birth to 6 months) obtained from line 5 mice showing a significant reduction in total body weight of muscle-specific dnOrai1 transgenic mice compared with WT littermate controls (n=5 female mice with both WT and dnOrai1). *p<0.01 compared to WT by Student's t-test. (b) Average (±s.e.m.) muscle weight of TA, EDL, and SOL in line 5 dnOrai1 transgenic mice (n=5 mice) compared to that of WT littermate controls (n=7 mice). *p<0.05 compared to corresponding WT control by Student's t-test. (c) Representative dystrophin immunostaining (top panel) and H&E images (bottom panel) of TA muscle cross sections from WT and line 5 dnOrai1 mice. Scale bars=100μm. (d) Average (±s.e.m.) fiber cross-sectional area calculated from dystrophin-stained TA muscle (as shown in c) from WT (n=4) and line 5 dnOrai1 mice (n=3). *p<0.05 by Student's t-test. (e) Representative silver-stained polyacrylamide gel images of myosin heavy chain analysis from TA, EDL SOL and FDB of three different WT and line 5 dnOrai1 mice. Molecular weight markers are indicated to the right of each gel. (f) Stacked bar graph showing average fast IIa, IIx, IIb and slow type I fibre composition in WT and line 5 dnOrai1 transgenic mice (n=3-5 for both WT and dnOrai1). No significant difference in fiber type composition was observed between WT and dnOrai1 mice.