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(A) Western blot showing overexpression of Sirt4. (B–F) ATP levels in Sirt4 overexpressing or knockdown cells (HEK293T, HHL-human hepatocytes, HEPG2 and C2C12 myotubes), as indicated. (G, H) ATP levels in wild-type and Sirt4KO (G) liver and (H) muscles under fed and fasted conditions. Statistical significance was calculated using student's t-test: * p < 0.05, ** p < 0.01, *** p < 0.001 or as indicated. Error bars indicate mean values ± SEM.
