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. 2013 Nov 6;288(51):36361–36371. doi: 10.1074/jbc.M113.522896

TABLE 1.

Kinetic parameters of P. falciparum and H. sapiens AsnRSs the ATP/PPi exchange reaction

Results represent averages of at least three independent experiments.

AsnRS ATP
 Asn
Km kcat kcat/Km Km kcat kcat/Km
μm s−1 s−1 μm−1 10−3 μm s−1 s−1 μm−1 10−3
P. falciparum 740 ± 240 10.1 ± 3.37 13.6 40 ± 40 4.2 ± 2.63 105
H. sapiens 970 ± 320 5.0 ± 1.58 5.2 460 ± 150 5.7 ± 1.09 12.4
E. coli (34) 500 2.2 4.4 40 2.8 70
CHO cells (35) 60