FIGURE 11.
Identification of base pairing of the wild-type SRP RNA by NMR spectra. A, two-dimensional NOESY of wild-type SRP RNA in H2O. Selected assignments are labeled in the spectrum. The NOEs (C17H41-G93H1 and C17H42-G93H1) provide direct evidence that C17 and G93 form a canonical GC base pair. B, imino-imino region in two-dimensional NOESY of WT SRP RNA in H2O. The lines indicate sequential imino proton connectivities. The inset in B shows the sequence and secondary structure of wild-type SRP RNA. The assignments and lines are colored by subdomain as in the inset.