FIGURE 4.

NoxA1ds disrupts Nox1-NOXA1 interaction. FRET between Nox1-YFP- and NoxA1-CFP-transfected COS22 cells in the presence or absence of 10 μm NoxA1ds or SCRMB was evaluated. Relative fluorescence of CFP is green, and YFP is red. Traces below the images indicate fluorescent intensities of CFP and YFP below the arrow overlaid on each cell. A, transfected COS22 cells were treated with vehicle for 1 h prior to imaging cells; photobleaching of Nox1-YFP was complete and resulted in a concomitant increase in CFP fluorescence. B, transfected COS22 cells were treated with 10 μm SCRMB peptide for 1 h prior to imaging cells; photobleaching of Nox1-YFP was complete and also resulted in a concomitant increase in CFP fluorescence. C, transfected COS22 cells were treated with 10 μm NoxA1ds peptide for 1 h prior to imaging cells; photobleaching of Nox1-YFP was complete but did not result in a concomitant increase in CFP fluorescence. D, quantification of FRET efficiency from images A–C. Values expressed as n = 8, three separate experiments; ***, p < 0.001 by one-way ANOVA and Bonferroni post-test.