FIGURE 5.

Structure of the GmSAT CoA binding site. A, stereoview of the CoA binding site along the left-handed β-helix domain. Surfaces corresponding to monomers are colored differently. The position of serine from the GmSAT-serine complex is shown for reference. The β1c-β2c loop is disordered in the GmSAT-CoA complex. B, GmSAT active site view. Residues interacting with CoA are shown, with an asterisk indicating residues from the adjacent monomer. The position of serine from the GmSAT-serine complex is shown for reference. C, C-terminal view of GmSAT showing the location of Arg²⁵³ (blue surface) in each monomer. CoA molecules are shown as space-filling models.