FIGURE 1.

Expression level of miR-200b shows an inverse relationship with CAGE. A, expression level of miR-200b in each cell line was determined by qRT-PCR. Each value represents an average obtained from three independent experiments. Data are expressed as a mean ± S.D. **, p < 0.005; ***, p < 0.0005. B, cell lysate prepared from each cell line was subjected to Western blot analysis. C, indicated cell line was treated with or without celastrol (100 nm) or taxol (100 nm) for various time intervals. The expression level of miR-200b was determined by qRT-PCR. Each value represents an average obtained from three independent experiments. Data are expressed as a mean ± S.D. ***, p < 0.0005; ###, p < 0.0005. Comparison was made between the cells treated with celastrol or taxol and the cells untreated. *** denotes statistical significance in response to celastrol. ### denotes statistical significance in response to taxol. D, same as C except that Western blot analysis was performed.