FIGURE 5.

Effect of miR-200b on invasion potential of cancer cells involves expression regulation of CAGE. A, SNU387^R, SNU387^R-taxol, Malme3M^R, Malme3M^R-taxol, AGS^R, or MDA-MB231 cells were transfected with control vector (NC, 1 μg) or miR-200b construct (1 μg). 48 h after transfection, miRNAs were isolated, and qRT-PCR was performed. miRNAs were also isolated from SNU387, Malme3M, AGS, or MCF-7 cells and subjected to qRT-PCR. **, p < 0.005; ***, p < 0.0005. B, same as A except that invasion potential was measured as described. C, SNU387 or Malme3M cells were transfected with control inhibitor (Ctrl. Inh.) (200 nm) or miR-200b inhibitor (200 nm). 48 h after transfection, invasion potential was measured (left panel). miRNAs were isolated and subjected to qRT-PCR (middle panel). Cell lyastes were isolated and subjected to Western blot analysis (right panel).