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pcsk7 is expressed in the head region, and pcsk7 morphant fish have cranial developmental defects. pcsk7 expression (dark blue) was analyzed with RNA in situ hybridization in wild-type zebrafish larvae at 2 dpf. A, pcsk7 antisense probe. B, negative control (pcsk7 sense probe). Right-hand panels show hematoxylin-eosin-stained transverse sections of zebrafish cranial region of RC (C) and pcsk7 e3 morphant (D) (3 dpf).
