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. 2013 Nov 6;288(51):36676–36690. doi: 10.1074/jbc.M113.508267

FIGURE 1.

FIGURE 1.

CID swapping changes the CTD-binding specificity of Nrd1. A, recruitment of Nrd1(CIDRtt103) on the PMA1 gene was monitored by ChIP assay. Nrd1(CIDRtt103) resembles Rtt103 in the recruitment pattern. Numbers below each lane refer to PCR primers as shown. B, recruitment of Nrd1(CIDRtt103) (either WT or Δrtt103 background) and Nrd1ΔCID was analyzed on the PMA1 genes by ChIP-quantitative PCR. Positions of PCR primers for PMA1 are as in A. C–E, recruitment of Nrd1(CIDRtt103) (either WT or Δctk1 background) and Nrd1ΔCID was analyzed by ChIP-qPCR. Positions of PCR primers are shown below. C, ADH1 gene. D, SNR13 gene. E, SNR33 gene. F, Nrd1(CIDRtt103) gains the ability to bind to the Ser(P)-2 CTD of RNApII as revealed by co-IP/Western blot analyses. All Nrd1 variants analyzed here were C-terminal TAP tagged for immunoprecipitation with IgG beads. Error bars represent S.E. for at least three independent experiments.