FIGURE 3.

Role of NFκB and STAT3 in THP-1 cell signaling. A, THP-1 cells were incubated with 40 μg/ml AS exosomes for the indicated length of time at 37 °C in the absence or presence of the JAK2 inhibitor P6 (2 μm), the JAK1 inhibitor AG490 (20 μm), or the specific NFκB inhibitor parthenolide (10 μm). Dimethyl sulfoxide (DMSO) served as solvent control. Cell lysates were prepared and analyzed by Western blotting with the indicated primary antibodies followed by peroxidase-conjugated secondary antibodies and ECL detection. B, RT-PCR analysis was performed on mRNAs isolated from cells treated in A. Error bars, S.E. C, THP-1 cells were incubated with exosomes as described above in the presence or absence of neutralizing antibodies to IL-6 and IL-6 receptor (each 1 μg/ml). Cell lysates were prepared and analyzed by Western blotting as described. ***, p < 0.001; **, p <0.01; *, p <0.05.