FIGURE 6.

TLR-specific antibody blocking and exosome signaling in mouse dendritic cells. A, antibodies to TLR2 (T2), TLR4 (T4), or in combination (each 3 μg/ml) were added to THP-1 cells followed by stimulation with AS exosomes. After 48 h mRNA was prepared, and the level of IL-1β and IL-6 was determined by RT-PCR. Data from two representative experiments of n = 4 are shown. STAT3 phosphorylation was examined by Western blotting. B, dendritic cells (mDCs) or macrophages (mMΦ) from B6 WT or MyD88^−/− mice were matured from bone marrow cells and stimulated with AF exosomes or TLR agonists LPS and P3C4. After a 48-h culture supernatants were collected, and the levels of TNF-α and IL-12 were determined. Error bars, S.E.