FIGURE 10.

Cleavage of the identified substrates in MPP⁺-stereotaxic injection model. A, at 3 days post-injection, the contralateral (C) and ipsilateral (I) side of tissue samples (substantia nigra pars compacta in midbrain) obtained from the MPP⁺-stereotaxic injection rats were processed for immunoblot analyses using the indicated antibodies that recognize endogenous proteins. TH was used to assess the extent of dopaminergic neurodegeneration, and fodrin and GAPDH were used as a calpain activation and loading control, respectively. B, after normalization to GAPDH, relative change in fold intensity of the indicated protein was determined over the full-length protein in the contralateral side (for ASNA1 and OPTN; value = 1) or the total fragments of the ipsilateral side (for PERI; value = 1). Each bar represents the mean ± S.E. from four independent samples. ***, p < 0.001.