FIGURE 8.

Changes in cellular distribution pattern and mRNA levels of the identified substrates in MPP⁺-induced cell death. A, immunofluorescent localization of each protein was measured in MN9D cells treated with 50 μm MPP⁺ for 36 h in the presence or absence of 50 μm calpeptin. Scale bars represent 5 μm. B, semi-quantitative RT-PCR was performed using mRNA prepared from MN9D cells treated with 50 μm MPP⁺ for the indicated time periods. Levels of 18 S rRNA were used as loading controls. Relative ratio of mRNA for ASNA1, OPTN, or PERI was calculated over the untreated controls (value = 1) after normalization to 18 S rRNA. All values represent the means ± S.E. from three independent experiments. Note that no significant changes in mRNA levels were detected. Cont., control.