TABLE 2.
Summary of proteins further identified after incubation with recombinant calpain
| Spot | Protein namea | Accession numberb | Molecular mass/pIc | Foldd | MOWSE scoree,f | Masses matchedf,g | Coveragef |
|---|---|---|---|---|---|---|---|
| Da | % | ||||||
| H23 | Ras-GTPase-activating protein-binding protein 1 | P97855 | 51,829/5.4 | 5.55 | 1.84E + 15 | 22 (11) | 48.8 |
| H19 | GMP synthase (glutamine-hydrolyzing) | Q3THK7 | 76,724/6.3 | 8.58 | 5.75E + 07 | 13 (7) | 23.4 |
| H17 | Stress-induced phosphoprotein 1 | Q60864 | 62,583/6.4 | 4.09 | 9.56E + 06 | 18 (9) | 36 |
| H18 | ATPase ASNA1 | O54984 | 38,823/4.8 | 0.38 | 1.00E + 09 | 14 (8) | 37.6 |
| H16 | Optineurin | Q8K3K8 | 67,018/5.2 | 0.17 | 4.19E + 13 | 35 (12) | 39 |
| H4 | Peripherin | P15331 | 54,268/5.4 | 0.23 | 4.78E + 08 | 21 (10) | 44 |
a Each protein spot in Fig. 2 was identified based on mass spectra of tryptic peptides obtained by MALDI-TOF mass spectrometry.
b Accession numbers from SwissProt data base (2012.12.3).
c Theoretical molecular mass and pI.
d Values present change of spots intensity in m-calpain-treated gel in comparison to untreated gel (value = 1).
e MOWSE scores were considered significant for identity (p < 0.05).
f These values were considered from SwissProt.
g The numbers in parentheses indicate percentages.