FIGURE 3.

Stimulation of AKR1B10 secretion by HSP90α. HSP90α alone or in combination with EGFP-AKR1B10 was delivered into cells, and AKR1B10 in medium was measured using ELISA as described under “Materials and Methods.” A, endogenous AKR1B10 secretion in HCT8 cells with ectopic expression of HSP90α. Con, control. B, secretion of exogenous EGFP-AKR1B10 fusion protein in 293T cells, alone or coexpressed with HSP90α. Data represent the mean ± S.D. from three independent assays. *, p < 0.01 compared with EFGP-AKR1B10 alone.