FIGURE 4.
Inhibition of AKR1B10 secretion by an HSP90α inhibitor. HCT-8 cells (2.5 × 105) were incubated for 12 h with the HSP90α inhibitor GA at the indicated concentrations and then fed with fresh serum-free medium for 30 min. The medium and cells were collected for sandwich ELISA, Western blot analysis, or immunoprecipitation. A, inhibition of HSP90α secretion. *, p < 0.05; **, p < 0.01 compared with vehicle control. B, dissociation of the HSP90 and AKR1B10 complexes by GA. α-B10, anti-AKR1B10 antibody.