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. 2013 Oct 30;155(1):89–97. doi: 10.1210/en.2013-1556

Figure 3.

Figure 3.

TSPO deletion in Leydig and Sertoli cells does not affect spermatogenesis. A, Immunohistochemical (IHC) localization showing complete absence of TSPO in Leydig and Sertoli cells of TSPOcΔ/Δ testes. Hematoxylin and eosin (H&E) staining showing unaltered seminiferous tubule morphology and spermatogenesis in TSPOcΔ/Δ testes (n = 5). Scale bars, 50 μm. B, Western blot showing absence of TSPO in TSPOcΔ/Δ testis tissue (n = 5); β-actin is shown as the loading control. C, Cauda epididymal sperm counts were not significantly different between TSPOfl/fl and TSPOcΔ/Δ mice (mean ± SEM; n = 5/group). D–F, Testis cDNA from TSPOfl/fl and TSPOcΔ/Δ mice examined for amplification products from exons 1 and 2 [250 bp] (D); exons 2 and 3 [241 bp] (E); exons 3 and 4 [424 bp] (F); exons 1–4 [711 bp in TSPOfl/fl and 361 bp in TSPOcΔ/Δ]. For all RT-PCR, glyceraldehydes-3-phosphate dehydrogenase was used as a control (CON).