Figure 6.

A) Acute CS had time-dependent effects on rhythms of inflammatory cell influx in mouse lungs. Acute (10 d) air or CS exposure was performed as described in Materials and Methods. At the end of CS or air exposure, lungs were harvested every 6 h for 42 h, beginning at ZT12 on the day following the last CS exposure. The number of total cells in BAL fluid from acute air- or CS-exposed mice was determined. At least 500 cells in the BAL fluid were counted with a hemocytometer to determine the number of neutrophils (i), macrophages (ii), and total cells (iii) on cytospin slides stained with Diff-Quik. Data from air- and CS-exposed lungs are shown as means ± se (n=3–4/group) for each time point. B) Chronic CS had time-dependent effects on rhythms of inflammatory cell influx in CS-exposed mouse lung. Environmental tobacco/chronic CS exposure (6 mo air or CS) was performed as described in Materials and Methods. On the day after the last exposure, lungs were harvested every 6 h for 24 h beginning at ZT0, and then at ZT6, ZT12, ZT18, and ZT24. The number of total cells in BAL fluid from chronic air- or CS-exposed mice was determined. At least 500 cells in the BAL fluid were counted with a hemocytometer, to determine the number of neutrophils (i), macrophages (ii), and total cells (iii) on cytospin slides stained with Diff-Quik. Data from air- and CS-exposed are shown as means ± se (n=3–4/group) for each time point. *P < 0.05 vs. corresponding air-exposed mice.