Skip to main content
. 2014 Jan;28(1):395–407. doi: 10.1096/fj.13-235382

Figure 3.

Figure 3.

PGC-1α is a CRM-1 cargo protein. A) Immunoprecipitation of PGC-1α followed by Western blot analysis of CRM-1 (top panel). Immunoprecipitation of PGC-1α followed by Western blot analysis of PGC-1α (bottom panel) shown as control for PGC-1α precipitation. B) Western blot analysis of PCG-1α in nuclear (Nu) and cytoplasmic (Cyto) fractions derived in HUVECs transduced with scrambled (SCR) or Akt3 shRNA lentiviruses (Akt3-LV), or transfection with a CRM-1 expression vector. Histone H3 shown as a nuclear control, and Src is shown as a cytoplasmic control. C) Real-time PCR analysis of Sirt3 and MCAD expression in HUVECs transfected with scrambled control RNAi (SCR), Akt3 RNAi (Akt3i), or FLAG-CRM-1. *P < 0.05.