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. 2014 Jan;28(1):256–264. doi: 10.1096/fj.13-238741

Figure 2.

Figure 2.

Influence of PDH inhibitor (HIF stabilizer) AKB-6899 on gravin induction in normoxia. A) Validation of HIF-1α stabilization by AKB6899. HMEC-1 cells were exposed to indicated concentrations of AKB6899 for 6 h. Nuclear lysates were assessed for HIF-1a stabilization by Western blot relative to TATA binding protein as a control. Representative experiment from n = 3. B) Real-time quantitative PCR analysis of gravin mRNA expression in HMEC-1 cells following exposure to indicated concentrations of AKB6899 in normoxia. Data were calculated relative to the β-actin and are expressed as mean ± sem fold change compared with control, n = 3. *P < 0.01. C) Western blot analysis of gravin following incubation with indicated concentrations of AKB6899, with β-actin as a control. Representative experiment from n = 3.