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Decreased p38 pathway signaling in RKO livers. A) Western blot analysis was performed with samples from animals that were unfed overnight and given rapamycin before refeeding. These were the same samples that were used for MS. B) An independent set of mouse liver samples derived from WT and RKO mice unfed overnight and refed for 45 min without rapamycin was similarly analyzed. C) These same samples were analyzed for the phosphorylation of p38 substrates.
