Fig. 3.

Effect of prototypic inducers of drug-metabolizing enzymes on CYP2C22 expression. Rat hepatocytes in sandwich cultures were treated with media (Con), 40 μM β-napthoflavone (βNF), 1 mM phenobarbital (PB), 20 μM dexamethasone (Dex), 25 μM PCN, or 10 μM clofibrate (Clo) for 72 hours, after which the cells were harvested and analyzed for CYP2C22 mRNA and protein expression. (A) Western blot of representative samples from each treatment group. (B) Quantitative analysis of individual samples (n = 3). Values are expressed relative to the control group. *Significantly different from control, P < 0.05, Dunnett’s test.