Fig. 6.

Stimulated degradation of CYP2C22 protein. (A) Western blot of lysates from cells treated for 12 hours with 500 μM NOC18 or medium (Con) in the presence (CHX) or absence (Mock) of 20 μg/ml cycloheximide. (B) Quantitative analysis of the data in (A). *Significantly different control, P < 0.05, t test (n = 3). (C) Cycloheximide chase experiment. Hepatocytes were treated with medium (Con) or 500 μM DPTA for 2, 4, 6, or 8 hours in the presence or absence of 20 μg/ml cycloheximide, and CYP2C22 levels were measured by Western blotting (n = 3). Exponential curve fitting was performed using Kaleidagraph software (Synergy Software, Reading, PA).