Skip to main content
. 2014 Jan;348(1):174–191. doi: 10.1124/jpet.113.208264

TABLE 3.

Statistical analyses of dose-effect curves for training drugs or substitution with various compounds compared with saline availability as shown in the Fig. 1.

Treatment Drug Dose Interaction Post Hoc Test
Subjects trained with d-methamphetamine
 d-Methamphetamine vs. saline substitution F1,20 = 34.1; P = 0.002 F4,20 = 31.6; P < 0.001 F4,20 = 32.2; P < 0.001 0.032 mg/kg/inj, t = 6.22, P < 0.001
0.10 mg/kg/inj, t = 11.0, P < 0.001
0.32 mg/kg/inj, t = 2.27, P = 0.034
 WIN35,428 vs. saline substitution F1,20 = 8.08; P = 0.036 F4,20 = 7.49; P < 0.001 F4,20 = 7.67; P < 0.001 0.01 mg/kg/inj, t = 3.26, P = 0.004
0.032 mg/kg/inj, t = 5.31, P < 0.001
 Cocaine vs. saline substitution F1,20 = 6.36; P = 0.053 F4,20 = 5.27; P = 0.005 F4,20 = 5.35; P = 0.004 0.1 mg/kg/inj, t = 2.48, P = 0.022
0.32 mg/kg/inj, t = 4.72, P < 0.001
 d-Amphetamine vs. saline substitution F 1,20 = 26.4; P = 0.004 F4,20 = 22.0; P < 0.001 F4,20 = 22.1; P < 0.001 0.032 mg/kg/inj, t = 4.88, P < 0.001
0.10 mg/kg/inj, t = 9.39, P < 0.001
0.32 mg/kg/inj, t = 2.80, P = 0.012
 JHW007 vs. saline substitution F 1,20 = 3.40; P = 0.125 F4,20 = 0.0539; P = 0.994 F4,20 = 5.35; P = 0.712 NS
 AHN2-005 vs. saline substitution F 1,20 = 0.606; P = 0.472 F4,20 = 0.174; P = 0.949 F4,20 = 0.863; P = 0.503 NS
 AHN1-055 vs. saline substitution F 1,20 = 11.9; P = 0.018 F4,20 = 11.3; P < 0.001 F4,20 = 12.1; P < 0.001 0.1 mg/kg/inj, t = 6.77, P < 0.001
0.32 mg/kg/inj, t = 3.68, P = 0.001
 Remifentanil vs. saline substitution F 1,20 = 3.49; P = 0.121 F4,20 = 2.84; P = 0.051 F4,20 = 2.87; P = 0.050 0.00032 mg/kg/inj, t = 2.42, P = 0.025
0.001 mg/kg/inj, t = 3.09, P = 0.006
 dl-Methadone vs. saline substitution F 1,20 = 25.1; P = 0.004 F4,20 = 23.1; P < 0.001 F4,20 = 25.0; P < 0.001 0.032 mg/kg/inj, t = 6.38, P < 0.001
0.10 mg/kg/inj, t = 8.91, P < 0.001
0.32 mg/kg/inj, t = 2.11, P = 0.048
 Morphine vs. saline substitution F 1,20 = 40.0; P = 0.001 F4,20 = 21.9; P < 0.001 F4,20 = 24.0; P < 0.001 0.032 mg/kg/inj, t = 6.74, P < 0.001
0.10 mg/kg/inj, t = 9.53, P < 0.001
0.32 mg/kg/inj, t = 3.44, P = 0.002
 Heroin vs. saline substitution F 1,20 = 25.3; P = 0.004 F4,20 = 19.9; P < 0.001 F4,20 = 19.8; P < 0.001 0.0032 mg/kg/inj, t = 4.99, P < 0.001
0.01 mg/kg/inj, t = 8.47, P < 0.001
0.032 mg/kg/inj, t = 3.61, P = 0.002
 (+)-MK-801 vs. saline substitution F 1,20 = 24.1; P = 0.004 F4,20 = 18.9; P < 0.001 F4,20 = 19.5; P < 0.001 0.001 mg/kg/inj, t = 4.59, P < 0.001
0.0032 mg/kg/inj, t = 9.04, P < 0.001
 Memantine vs. saline substitution F 1,20 = 24.2; P = 0.004 F4,20 = 31.1; P < 0.001 F4,20 = 26.1; P < 0.001 0.1 mg/kg/inj, t = 5.46, P < 0.001
0.32 mg/kg/inj, t = 9.60, P < 0.001
 Ketamine vs. saline substitution F 1,20 = 14.2; P = 0.013 F4,20 = 10.7; P < 0.001 F4,20 = 10.7; P < 0.001 0.1 mg/kg/inj, t = 3.21, P = 0.004
0.32 mg/kg/inj, t = 6.96, P < 0.001
Subjects trained with heroin
 Heroin vs. saline substitution F1,20 = 26.6; P = 0.004 F4,20 = 25.3; P < 0.001 F4,20 = 28.5; P < 0.001 0.0032 mg/kg/inj, t = 6.57, P < 0.001
0.010 mg/kg/inj, t = 9.39, P < 0.001
0.032 mg/kg/inj, t = 2.47, P = 0.025
 d-Methamphetamine vs. saline substitution F 1,20 = 20.5; P = 0.006 F4,20 = 23.2; P < 0.001 F4,20 = 22.0; P < 0.001 0.032 mg/kg/inj, t = 5.37, P < 0.001
0.1 mg/kg/inj, t = 8.25, P < 0.001
0.32 mg/kg/inj, t = 2.91, P = 0.011
 WIN35,428 vs. saline substitution F 1,20 = 27.2; P = 0.003 F4,20 = 28.8; P < 0.001 F4,20 = 29.4; P < 0.001 0.01 mg/kg/inj, t = 6.80, P < 0.001
0.032 mg/kg/inj, t = 9.56, P < 0.001
 Cocaine vs. saline substitution F1,20 = 4.48; P = 0.088 F4,20 = 3.64; P = 0.022 F4,20 = 3.64; P = 0.022 0.32 mg/kg/inj, t = 4.01, P < 0.001
 d-Amphetamine vs. saline substitution F1,20 = 32.2; P = 0.002 F4,20 = 32.7; P < 0.001 F4,20 = 34.5; P < 0.001 0.032 mg/kg/inj, t = 6.88, P < 0.001
0.1 mg/kg/inj, t = 10.5, P < 0.001
0.32 mg/kg/inj, t = 3.08, P = 0.007
 JHW007 vs. saline substitution F1,20 = 0.253; P = 0.636 F4,20 = 15.3; P < 0.001 F4,20 = 4.38; P = 0.011 NS
 AHN2-005 vs. saline substitution F1,20 = 1.57; P = 0.266 F4,20 = 4.36; P = 0.011 F4,20 = 1.78; P = 0.173 1.0 mg/kg/injection, t = 2.20, P = 0.038
 AHN1-055 vs. saline substitution F1,20 = 12.7; P = 0.016 F4,20 = 10.7; P < 0.001 F4,20 = 11.5; P < 0.001 0.1 mg/kg/inj, t = 5.80, P < 0.001
0.32 mg/kg/inj, t = 5.02, P < 0.001
 Remifentanil vs. saline substitution F1,20 = 7.95; P = 0.037 F4,20 = 6.15; P = 0.002 F4,20 = 6.13; P = 0.002 0.00032 mg/kg/inj, t = 3.22, P = 0.004
0.001 mg/kg/inj, t = 4.81, P < 0.001
 dl-Methadone vs. saline substitution F1,20 = 36.8; P = 0.002 F4,20 = 23.0; P < 0.001 F4,20 = 25.1; P < 0.001 0.032 mg/kg/inj, t = 6.38, P < 0.001
0.1 mg/kg/inj, t = 9.82, P < 0.001
0.32 mg/kg/inj, t = 3.46, P = 0.003
 Morphine vs. saline substitution F1,20 = 33.9; P = 0.002 F4,20 = 24.2; P < 0.001 F4,20 = 21.8; P < 0.001 0.032 mg/kg/inj, t = 6.10, P < 0.001
0.1 mg/kg/inj, t = 9.28, P < 0.001
0.32 mg/kg/inj, t = 3.01, P = 0.007
 (+)-MK-801 vs. saline substitution F1,20 = 0.560; P = 0.488 F4,20 = 5.82; P = 0.003 F4,20 = 3.65; P = 0.022 0.01 mg/kg/inj, t = 2.22, P = 0.037
 Memantine vs. saline substitution F1,20 = 3.16; P = 0.135 F4,20 = 6.36; P = 0.002 F4,20 = 1.88; P = 0.154 0.032 mg/kg/inj, t = 2.85, P = 0.009
 Ketamine vs. saline substitution F1,20 = 1.07; P = 0.348 F4,20 = 4.70; P = 0.008 F4,20 = 1.06; P = 0.403 NS
Subjects trained with ketamine
 Ketamine vs. saline substitution F1,20 = 12.9; P = 0.016 F4,20 = 10.8; P < 0.001 F4,20 = 10.3; P < 0.001 0.1 mg/kg/inj, t = 2.92, P = 0.008
0.32 mg/kg/inj, t = 6.84, P < 0.001
 d-Methamphetamine vs. saline substitution F1,20 = 14.9; P = 0.012 F4,20 = 26.5; P < 0.001 F4,20 = 13.6; P < 0.001 0.032 mg/kg/inj, t = 5.95, P < 0.001
0.1 mg/kg/inj, t = 5.56, P < 0.001
 WIN35,428 vs. saline substitution F1,20 = 9.14; P = 0.029 F4,20 = 22.8; P < 0.001 F4,20 = 9.36; P < 0.001 0.01 mg/kg/inj, t = 3.45, P = 0.004
0.032 mg/kg/inj, t = 5.52, P < 0.001
 Cocaine vs. saline substitution F1,20 = 6.49; P = 0.051 F4,20 = 5.90; P = 0.003 F4,20 = 5.35; P = 0.004 0.1 mg/kg/inj, t = 2.45, P = 0.023
0.32 mg/kg/inj, t = 4.75, P < 0.001
 d-Amphetamine vs. saline substitution F1,20 = 17.9; P = 0.008 F4,20 = 40.9; P < 0.001 F4,20 = 16.1; P < 0.001 0.032 mg/kg/inj, t = 4.75, P < 0.001
0.1 mg/kg/inj, t = 7.59, P < 0.001
 JHW007 vs. saline substitution F1,20 = 0.644; P = 0.459 F4,20 = 4.47; P = 0.010 F4,20 = 0.414; P = 0.797 NS
 AHN2-005 vs. saline substitution F1,20 = 0.609; P = 0.470 F4,20 = 1.02; P = 0.421 F4,20 = 2.00; P = 0.133 NS
 AHN1-055 vs. saline substitution F1,20 = 3.66; P = 0.114 F4,20 = 9.07; P < 0.001 F4,20 = 0.932; P = 0.465 NS
 Remifentanil vs. saline substitution F1,20 = 1.24; P = 0.317 F4,20 = 3.49; P = 0.026 F4,20 = 1.57; P = 0.221 NS
 dl-Methadone vs. saline substitution F1,20 = 2.16; P = 0.201 F4,20 = 7.49; P < 0.001 F4,20 = 0.419; P = 0.793 NS
 Morphine vs. saline substitution F1,20 = 1.34; P = 0.300 F4,20 = 1.89; P = 0.151 F4,20 = 1.32; P = 0.295 NS
 Heroin vs. saline substitution F1,20 = 0.746; P = 0.427 F4,20 = 0.904; P = 0.480 F4,20 = 1.64; P = 0.203 NS
 (+)-MK-801 vs. saline substitution F1,20 = 10.9; P = 0.021 F4,20 = 30.1; P < 0.001 F4,20 = 15.9; P < 0.001 0.001 mg/kg/inj, t = 4.15, P = 0.001
0.0032 mg/kg/inj, t = 6.39, P < 0.001
0.01 mg/kg/inj, t = 2.37, P = 0.037
 Memantine vs. saline substitution F1,20 = 9.98; P = 0.025 F4,20 = 27.5; P < 0.001 F4,20 = 11.5; P < 0.001 0.1 mg/kg/inj, t = 3.88, P = 0.002
0.32 mg/kg/inj, t = 5.75, P < 0.001

NS, not statistically significant; inj, injection.