Fig. 6.

SGK2-dependent glucose production. (A) ShP51 cells were treated with simvastatin (SIM, 10 μM) or DMSO (control) for 1, 3, and 5 hours (n = 3). The relative expression of G6Pase mRNA was measured by qRT-PCR and was normalized to endogenous glyceraldehyde-3-phosphate dehydrogenase using the comparative cycle threshold method. Values are scored as fold-changes relative to 1-hour DMSO-treated cells. (B) ShP51 cells were transfected with SGK2 siRNA or control siRNA, followed by treatment with simvastatin (SIM, 10 μM) or DMSO (control) for 4 hours (n = 4). The total glucose contents were measured using a glucose colorimetric/fluorometric assay kit and were normalized to the total protein contents. Statistical analysis was done by one-way ANOVA; *P < 0.05; ***P < 0.001.