Table 1.
Primers used in this study.
| Protein | Primer | Primer sequence (5′-3′) | Ref. |
|---|---|---|---|
| 16S ribosomal RNA | 16S rDNA-F | CTTGACGTTAGCGACAGAAGAA | ** |
| 16S rDNA-R | CGCTTTACGCCCAGTAATTC | ||
| Acetyl-CoA carboxylase subunit beta | accD-F | TTGCTGGTCGTCGTGTTATT | ** |
| accD-R | TTTAGCCATCAAACCACCAA | ||
| Cell division protein FtsZ | ftsZ-F | CGGATGTTCGTACGGTAATG | ** |
| ftsZ-R | CAAGCAATGGGCTTGAGATA | ||
| RNA polymerase sigma factor RpoD | rpoD-F | AAGCCGAAGAAATTCGCTTA | ** |
| rpoD-R | GCAAGATCTGATTCGCTCAA | ||
| Glyceraldehydes-3-phosphate dehydrogenase | gapA-F | TTTGTTTTCCGTGCATCTGT | * |
| gapA-R | GTTGAAACGACCGTGAGTTG | ||
| DNA polymerase I | polA-F | TTCCTGGCATTGGTGATAAA | * |
| polA-R | AAACCAAGAGGCGCAATATC | ||
| LitR family transcriptional regulator | litR-F | GCTCAATCCCTTCAGCAAAC | ** |
| litR-R | GTGGTTTGAATGGAGCACCT | ||
| Hemolysin Vah2 | vah2-F | GAAATTTCCATGACGCCTTC | * |
| vah2-R | ACGCCTTTCACAACTGTTCC | ||
| Hemolysin Vah5 | vah5-F | CACAAGACAGTCCGGTTCCT | * |
| vah5-R | ATTGCACCAAAAGCCAATTC | ||
| Hemolysin III | hlyIII-F | AATGGCTGTGATCTGGGCTA | * |
| hlyIII-R | AACCGATAACCAACCCATGA | ||
| Type VI secretion protein VasA | vasA-F | CAACGCCTGTCGGTAATTCT | * |
| vasA-R | TGGGGTTCATTGTCAGTTCA | ||
| Putative type I toxin secretion system | tolC-F | CTTTCTGAGCTTGGGTTTCG | * |
| tolC-R | GCACAATTTCGCTCATCAGA | ||
| Type II secretion pathway protein D | epsD-F | GTGCACCGTAATCACCCTCT | * |
| epsD-R | CCATGGTGGGCTTAGAAGAA | ||
| Flp pilus assembly protein | tadA-F | ACTTTTCGGCTACCATCGTG | * |
| tadA-R | CTGCCCTTAGAAGCAATTCG | ||
| Elongation factor 1-beta | EF1β-F | TGCCCCTCCAGGATGTCTAC | *** |
| EF1β-R | CACGGCCCACAGGTACTG | ||
| β-actin | β-actin-F | CCAAAGCCAACAGGGAGAAG | *** |
| β-actin-R | AGGGACAACACTGCCTGGAT | ||
| 18S ribosomal RNA | 18S rDNA-F | TGTGCCGCTAGAGGTGAAATT | *** |
| 18S rDNA-R | CGAACCTCCGACTTTCGTTCT | ||
| Toll-like receptor 5, solid form | TLR5S-F | CCTTGGATCTCCATGGTGTCA | **** |
| TLR5S-R | TGGTTTTGGGTACTTTTCCATTATG | ||
| Tumor necrosis factor alpha | TNFα-F | AATACAACCCCAGTGGATCG | **** |
| TNFα-R | TGTCCTTGTTAGGATGCTTGG | ||
| Interleukin 1-beta | IL-1β-F | GCTGGAGAGTGCTGTGGAAGA | **** |
| IL-1β-R | TGCTTCCCTCCTGCTCGTAG | ||
| Interleukin 6 | IL-6-F | ACCAACAGTTTGTGGAGGAGTT | **** |
| IL-6-R | AGCAAAGAGTCTTGGAGAGGTG | ||
| Interleukin 8 (CXCL8) | IL-8-F | ATTGAGACGGAAAGCAGACG | **** |
| IL-8-R | CGCTGACATCCAGACAAATCT | ||
| Interleukin 12 | IL-12-F | CTGAATGAGGTGGACTGGTATG | **** |
| IL-12-R | ATCGTCCTGTTCCTCCG | ||
| Interferon alpha | IFNα-F | TGGGAGGAGATATCACAAAGC | **** |
| IFNα-R | TCCCAGGTGACAGATTTCAT | ||
| Complement component 3 | C3-F | TCCCTGGTGGTCACCAGTACAC | ***** |
| C3-R | ATGATGGTGGACTGTGTGGATC |