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Lodoxamide and bufrolin are high potency agonists at both human and rat GPR35, whereas other ligands display substantial species ortholog selectivity. The ability of varying concentrations of lodoxamide (A), bufrolin (B), zaprinast (C) amlexanox (D), doxantrazole (E), and pemirolast (F) to promote interactions between β-arrestin-2 and either human GPR35a or rat GPR35 in BRET-based assays is shown.
