Fig. 5.

RNA polymerase chain reaction (PCR) analysis of the expression of genes related with BA signaling. Levels of mRNAs for FGF15, ileal bile acid-binding protein (IBABP), ASBT, SHP (B), FXR, PXR, CAR, and VDR (A) were determined in SI for adult male WT and LCN mice. RNA-PCR was performed as described in Materials and Methods. RNA samples were prepared from intestinal epithelial cells of individual mice. The results are shown as relative levels in arbitrary units, with the WT group as 1 (means ± S.D; n = 4). *P < 0.05; **P < 0.01, compared with the corresponding WT group (Student’s t test). The results are typical of three separate experiments; no sex difference was observed (data not shown).