Fig. 2.

Effects of NECA on the expression of members of the Jun family transcription factors in cells with adenosine-dependent regulation of VEGF. Real-time reverse-transcription polymerase chain reaction analysis of mRNA encoding c-Jun, JunB, and JunD in (A) HMEC-1 cells, (B) HMC-1 cells, (C) mCSC cells, and (D) LLC cells incubated in the absence (basal) or presence of 10 μM NECA for 60 minutes. Values are normalized to β-actin mRNA expression and are expressed as the average of two determinations made in triplicate.