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. 2014 Jan;85(1):62–73. doi: 10.1124/mol.113.088567

Fig. 5.

Fig. 5.

Effects of inhibitors of protein kinases A and C on A2B receptor-dependent increase in VEGF secretion and JunB protein levels. (A) VEGF secretion from HMEC-1 cells incubated in the absence (basal) or presence of 10 μM NECA, or in the presence of NECA and 1 μM H-89, 100 μM Rp-cAMPS, or 1 μM Gö6983 for 6 hours. Values are presented as mean ± S.E.M. (n = 3). (B) Western blot analysis of JunB protein levels in HMEC-1 cells incubated in the absence (basal) or presence of 10 μM NECA, or in the presence of NECA and 1 μM H-89, 100 μM Rp-cAMPS, or 1 μM Gö6983 for 3 hours. Arrows indicate positions of JunB and β-actin, the latter used as a loading control. A representative blot of three experiments is shown. (C) Graphic representation of data shown in B quantified by densitometry and expressed as relative density of JunB bands normalized to corresponding β-actin bands.