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. 2014 Jan;85(1):62–73. doi: 10.1124/mol.113.088567

Fig. 6.

Fig. 6.

Effects of PLC and MEK inhibition on VEGF secretion and JunB levels in HMEC-1 cells. (A) Effect of the PLC inhibitor U73122 (●) and its inactive control analog U73343 (○) on VEGF secretion from cells stimulated with 10 μM NECA. In the absence of inhibitors, 10 μM NECA increased concentrations of VEGF in the medium from 47 ± 1 to 138 ± 1 pg/106 cells. Values are presented as mean ± S.E.M. (n = 6). (B) Effect of increasing concentrations of the MEK inhibitor U0126 (●) and its inactive control analog U0124 (○) on VEGF secretion from cells stimulated with 10 μM NECA. Values are presented as mean ± S.E.M. (n = 3). (C) Western blot analysis of JunB protein levels in HMEC-1 cells incubated in the absence (basal) or presence of 10 μM NECA, or in the presence of NECA and 10 μM U73122, 10 μM U73343, 1 μM U0126, or 1 μM U0124 for 3 hours. Arrows indicate positions of JunB and β-actin, the latter used as a loading control. A representative blot of three experiments is shown. (D) Graphic representation of data shown in C quantified by densitometry and expressed as relative density of JunB bands normalized to corresponding β-actin bands.