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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: Nature. 2013 Dec 12;504(7479):277–281. doi: 10.1038/nature12783

Figure 2. Skin reconstitution assays.

Figure 2

(a) Experimental set up for (b-f). (b-c) Grafts immunostained with DAPI counterstain (blue). SG: sebaceous gland. Arrows: APM. (d-f) Contribution of PDGFRaH2BeGFP cells to dermal compartments. (g) Experimental set up for (h-k). (h) Macroscopic views of grafts. (i) Contribution of GFP+ cells to grafts. Arrows: GFP+ DP. (j) Hair follicles per graft. (k) GFP+ DP per 120 microns graft. N=3 biological replicates per experiment. *P <0.05; **P <0.005. Horizontal whole mounts shown. Scale bars: (b-c) 40 μm (e) 30 μm (h) 2.5mm (i) 50 μm.