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Genotypic analysis of WT, SKO and DKO cell lines. Southern blot analysis of SacI-digested genomic DNA (∼5 μg) from diploid wild-type trypanosomes (WT), ΔUMPS::HYG (SKOHYG); ΔUMPS:PAC (SKOPAC); and ΔUMPS::HYG/ΔUMPS::PAC (DKO) using a probe against the 5′-UTR of UMPS. A schematic representation of the OMPDC locus and its gene replacements is shown to the right of the blot.
