Figure 8.

Utilization of uracil and production of orotate. Cells were cultured in HMI9T at an initial seeding density of 1 × 10³ cells ml⁻¹, with medium harvested after 72 h and analysed by HPLC. A and B show the amount of uracil utilized and orotate produced by WT (closed bars), DKO (open bars) and DKO^M (gray bars) cells. The respective cell densities at this stage were 7.2 × 10⁶ cells ml⁻¹ (WT) and 3.2 × 10⁶ cells ml⁻¹ (DKO and DKO^M). The initial concentration of uracil in the medium was 4.43 ± 0.26 μM.